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In this research, a lethal concentration of levofloxacin was utilized to treat K. pneumoniae. This action led to the formation of a noticeable plateau of surviving levofloxacin-tolerant persisters. We then collected bacterial samples from K. pneumoniae persisters at five distinct time points within their formation and subsequent recovery, permitting transcriptome analysis. During the development of persisters, the ptsH gene's expression was observed to be elevated; however, it was


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