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Real-time PCR and ELISA were utilized to quantify the relative expression levels of IDO, PD-L1, COX-2, and HGF genes, as well as the levels of IL-6 and TGF- cytokines in IFN-stimulated and unstimulated ASCs (both treated and untreated cells). A statistically significant increase (P0.05) in TGF- levels was observed in unstimulated ASCs that were treated with 10-8 M naltrexone (NTX) when compared to the control group. Elevated expression of IDO, PD-L1 genes, and IL-
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