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Finally, the expression of hsa circ 0072309 in GC tissues and cultured cells was validated, and the potential relationship between its expression profile and clinical manifestations was investigated. The research program also encompassed experiments related to both gain- and loss-of-function, and molecular interactions. 7 differentially expressed circRNAs, 13 DEMIs, and 17 DEMs were discovered in a preliminary screening. The two DEMIs (hsa miR-34a-3p