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An investigation into the correlation between OTUD1 and P16 was undertaken, utilizing Western blot and immunohistochemistry as the methodologies. Increased OTUD1 expression, as seen in our findings, was associated with a reduction in TNBC cell proliferation and amplified sensitivity to doxorubicin. Experiments designed to rescue the system confirmed that the chemosensitizing impact of enhanced OTUD1 expression was nullified by the inhibition of P16. Our results emp