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To develop and characterize a new type of plasma rich in growth factors (PRGF) membrane for patients in which immune system is involved in the disease etiology. Blood from three healthy donors was collected to obtain the different fibrin membranes by PRGF technology. PRGF obtained volumes were activated and divided into two groups PRGF membrane (mPRGF) obtained after incubation at 37°C for 30min (control); and is-mPRGF mPRGF obtained after incubation for 30min at 56°C. The concentration of several growth factors, proteins, immunoglobuli