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Quantitative real-time PCR (qRT-PCR), western blot, and immunohistochemistry were employed to detect LITAF expression. The research also sought to understand how LITAF influenced the forkhead box O 1 (FOXO1)-sirtuin 1 (SIRT1) signaling pathway. A xenograft mouse model was further used to study the in-vivo effects of LITAF. Tumor tissues showed diminished LITAF expression, which was statistically associated with prognostic markers including pathological stage and the presence
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