http://biotiny.com/index.php/a....-manuscript-donor-ac
The sequential AK3L1 response to the external urea perturbation proceeds through a series of thoroughly defined steps. Crowding agents, when incorporated, not only adjust the enzyme's peak activity to higher urea levels, but also improve the compactness of the domain, as revealed by fluorescence resonance energy transfer studies. The enzyme's global unfolding is preceded by local responses, including modulations in enzy
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