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Reversibly glycosylated polypeptide (RGP), a kind of hydrosoluble and plasmodesmal-associated protein found in plants, plays a crucial role in the development of pollen. A novel RGP 2 was isolated and identified from rape (Brassica napus L.) bee pollen. RGP2 was isolated and purified by ion-exchange column and gel filtration chromatography, and characterized by MALDI-TOF-MS, LC-MS, immunological histological chemistry, and transmission electron microscope. Our results indicated that the RGP2 is an acidic protein (pI=5.46) with the molec