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A diagnosis of Brucella canis infection is unequivocally determined through the use of the one-step BcSS-PCR technique. The results of the study compelled the veterinary authorities to draw up action plans to control this disease affecting dogs. The human and animal intestines may contain this protozoan. Diarrhea, along with other mild or severe intestinal complications, can result from the parasite's impact on both healthy and immunocompromised people. The ob