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The process of negative selection, acting as a self-antigen, decreases the number of WT1-specific T cell receptors (TCRs). A procedure is outlined for the generation of WT137-45-targeted TCRs from the human peripheral blood's mononuclear cells. We studied the expansion of WT1-specific T cell clones by employing two cycles of in-vitro stimulation using autologous WT137-45-pulsed dendritic cells and peripheral blood lymphocytes. We subsequentl
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