https://gsk429286ainhibitor.co....m/predictive-great-n
Among various homologous recombination (HDR) donor types, single-stranded DNA (ssDNA) donors are often preferential when introducing short sequence knock-ins or base substitutions, while linear dsDNA represents another approach. However, the difficulty in creating extended single-stranded DNA (ssDNA) makes it questionable whether it functions as the optimal homologous recombination donor template for inserting
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