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Female C57BL/6J mice, eight weeks of age, were used to establish ovariectomy (OVX)-induced estrogen deficiency-related osteoporotic mouse models and their corresponding sham control models. A micro-computed tomography (CT) scan was employed to ascertain the skeletal physical characteristics. The differentiation potential of bone marrow mesenchymal stem cells (BMSCs), derived from the OVX and sham groups, was studied using osteogenic or adipogenic induction medium. Aligning AP's impact, alizarin red S (ARS), alkaline phosphatase (ALP), and oil red O (ORO) sta
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