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ion with theobromine as feedstock through the N-demethylation at N-7 position. This study is the first to confirm that A. sydowii PT-2 and A. tamarii PT-7 degrade theobromine through N-demethylation and oxidation, respectively. A. sydowii PT-2 showed the potential application in 3-methylxanthine production with theobromine as feedstock through the N-demethylation at N-7 position. Effective communication is crucial to any doctor-patient consultation, not least in pregnancy where the outcome affects more than one person. While higher level