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These effects were also accompanied by inhibition of cell migration and invasion, indicating that miR-16 can have a significant role as liver cancer suppressor after zingiberene treatment. Luciferase reporter assay confirmed that miR-16, which was one of HCC downregulated miRs, directly targeted Cyclin B1 in HCC cells. The current study indicates miR-16/cyclin B1 axis might have significant applications as a therapeutic target for patients with liver cancer. The current study indicates miR-16/cyclin B1 axis might have significant applica