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65, 95% CI 0.53 to 0.8 could reduce the risk of disease infection. There was no statistical significance advantage to hand washing more than 10 times/day compared to 5-10 times/day (OR =0.86, 95% CI 0.70 to 1.06). Comparing hand washing ≤10 times/day with hand washing 10 times/day, increased hand washing was a protective factor against infection (OR =0.59, 95% CI 0.36 to 0.97). The more frequently hands were washed, the lower risk of disease. So far however, there is no high-quality evidence indicating the best range of hand washi