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© Copyright 2020 Springer Publishing Company, LLC.RNAs that are 5'-truncated versions of a longer RNA, but share the same 3' terminus can be generated by alternative promoters in transcription of cellular mRNAs or by ****ting RNA viruses. These truncated RNAs cannot be distinguished from the longer RNA by a simple two-primer RT-PCR because primers that anneal to the cDNA from the smaller RNA also anneal to - and amplify - the longer RNA-derived cDNA. Thus, laborious methods, such as northern blot hybridization, are used to distinguish
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