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Employing morphological and analytical methods, a study of the AXJ-M heat stress response was performed. Comparative genomic research uncovered intriguing facets of the genetic adaptability of AXJ-M strains in rigorous environments. The TMT quantitative proteomic approach, combined with parallel reaction monitoring (PRM), revealed differential protein expression patterns associated with both component systems. These proteins included those involved in ABC transporters, carbohydrate and amino ac