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Di-n-butyl phthalate (DBP), one of the most widely used plasticizers, has been listed as a priority pollutant because of its toxicity to both humans and animals. In this study, Pseudomonas sp. W1, isolated from activated sludge, was capable of degrading 99.88% of DBP (1000 mg L-1) within 8 days. We immobilized the W1 strain using Fe3O4 iron nanoparticles (IONPs) coated with poly-dopamine (PDA), and further evaluated its DBP degradation efficiency. The DBP degradation performance of W1 was improved by immobilization, exhibiting 99.69% of