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Employing a covalent immobilization technique, T2DM PRP proteins were bound to polycaprolactone (PCL) nanofibers, and the growth of endothelial cells on the resultant PCL-COOH-PRP material was investigated. T2DM PRP samples were analyzed for NO levels, which correlate with the cytotoxic effects of PRP, angiogenin, and VEGF. The results showed that the application of PCL-COOH-PRP nanofibers effectively removed the cytotoxic effects associated