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Out of this population, 50 (41.7%), 26 (52%), and 24 (48%) cases had infection, pityriasis versicolor, and seborrheic dermatitis, respectively. was found to be the most prevalent species (n=29, 58%), followed by (n=20, 40%), and (n=1, 2%). The epidemiologic study was indicative of the frequency of some species, such as and , in Isfahan, Iran. It can be concluded that direct PCR on clinical samples could be used as a simple, precise, effective, fast, and affordable method for research and even routine medical mycology laboratory studies.