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The catalytic performance of certain PtNi/MWCNTs collected during the staircase heating process was better than that of PtNi/MWCNTs produced via the conventional heating process.Flaviviruses including dengue virus and Zika virus encode a unique two-component NS2B-NS3 protease essential for maturation/infectivity, thus representing a key target for designing antiflavivirus drugs. Here, for the first time, by NMR and molecular docking, we reveal that curcumin allosterically inhibits the dengue protease by binding to a cavity with no overlap