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BLASTn comparisons of the generated sequences indicated a similarity of 98% to 100% with the corresponding sequences of Botrytis aclada. A phylogenetic tree, generated using MEGA110 and combining sequenced loci, shows a 99% bootstrap support clustering isolates JQ21AC03 and JQ21AC09 with B. aclada. The isolates JQ21AC03 and JQ21AC09 were determined to be B. aclada, according to the findings. The two strains' ability to cause disease in the onion cultivar i