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The results were validated quantitatively on 2D MPR images. An accuracy of 95.265±0.4551% was achieved through volumetric overlap computation. Through pairedt-test, at α=5%, statistical values were p=0.6769, and t=0.4169 which infer that there was no much significant difference. CONCLUSION The combination of different validation techniques was applied to check the robustness of colon segmentation method, and good results were achieved with this approach. Through quantitative validation, the results were accepted at α=5%. B