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The present study aimed to elucidate the developmental kinetics, growth potential, and viability of bovine embryos produced in vitro with ****ed semen. Bovine oocytes were fertilized in vitro using unsorted and X-sorted semen from the same Holstein bulls, and the kinetics of in vitro development were continuously monitored for 10 d through time-lapse cinematography. The blastocyst formation rate was lower in the X-sorted group than in the unsorted group (P less then 0.01), whereas the normal fertilization rate did not differ between groups