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As a result, we saw an upregulation of Cdk2, a downstream target of p21, indicating accelerated G1/S phase transition in the hearts of Hdac1-deficient zebrafish. Cdk1, a downstream component of both p21 and Cdc25 pathways, was demonstrably downregulated. Hdac1-deficient zebrafish embryos displayed a notable decrease in Cdc25 protein expression, leading to reduced levels of Cdk1 and suggesting an impediment in G2/M phase advancement. Our
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