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A comprehensive analysis of the ascorbic acid nano-emulsion was performed employing the DLS Zetasizer, zeta potential, transmission electron microscopy (TEM), and Fourier transform infrared spectroscopy (FT-IR) methods. Employing the broth microdilution technique, the antibacterial activity of ASC-NEs was examined, and subsequently, phenotypic and RT-PCR experiments were conducted to investigate the effect of sub-MIC levels on the expres
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