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Phosphorylation of Ser and Thr residues has the potential to impact the local and global structure of Tau.Neurofibrillary tangle (NFT), bundle of paired helical filaments in neurons is one of the defining features of Alzheimer's disease (AD) and their spreads well correlate with disease symptoms and progression of AD. Using the unusual insolubility, NFTs were partially purified and the antibodies were produced. Characterization of these antibodies and biochemical studies of tau in AD revealed that a hyperphosphorylated tau protein is the