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The diverse operational ranges of our method underscore its potential applicability to a broad array of bioanalytical procedures. In centimeter-scale ATPS, the distribution ratios of IgG and FITC, optimized using PEG 6000 Da and dextran 10000 Da in 10 mM PBS, were measured at 91682 and 0998, respectively. Millimeter-scale ATPS systems enhance analyte concentrations by 6067-fold. This is accomplished by utilizing a solution containing 15 weight