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01 U/mL (FA ) and 6.46 U/mL (WD ) and a maximum decrease of the adhesion by 37.5% (FA ) and 38.42% (WD ). The effect of heparin on P-selectin was tested using anti-P-selectin antibodies alone and in combination with heparin. Adding heparin after antibody treatment resulted in an additional reduction of 9.52% (FA ) and 7.12% (WD ). Together, we quantified heparin's antimetastatic effect and proved that it predominantly is related to the blockage of P-selectin. Our approach represents a valuable method to investigate the adhesion of pl