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Etoposide's influence on cell proliferation was evaluated using both an in vitro CCK-8 assay and an in vivo tumor-bearing mouse model. To assess the influence of etoposide on tumor cell migration and invasion, wound healing and Boyden chamber assays were employed. The impact of etoposide on the organization of microfilaments was illustrated by immunofluorescence staining, utilizing phalloidine as a marker. A half-life experiment and immunofluorescence staini