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P. jirovecii was detected in 19% [95% confidence interval (CI) 12-27%] of 3583 symptomatic and in 9% [95% CI 0-45%] of 140 asymptomatic adults. Among symptomatic adults, prevalence was 22% [95% CI 12-35%] by PCR and 15% [95% CI 9-23%] by microscopy. Seven percent of 435 symptomatic adults had PCR-detected Pneumocystis colonization without evidence of PCP [95% CI 5-10%, four studies]. One study established a qPCR cutoff of 78 copies/5μl of DNA in 305 induced sputum samples to distinguish Pneumocystis colonization from microscopy-confirme