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Through the I-TASSER server, the 3D structures of the best CPP-Endostatin fusions were predicted. To validate the predicted models, the PROCHECK, ERRAT, Verify3D, and ProSA-Web servers were employed. PyMol, a molecular graphics system, was utilized to visualize the top-performing models. Based on the principal parameters for selecting optimal CPPs in endostatin delivery, the analysis of endostatin fusions with four CPPs – Cyt c-ss-M