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The anti-vascular effect could only be observed when laser and ultrasound were properly synchronized in vivo. CONCLUSION PUT is more efficient when the laser-induced photoacoustic wave overlays the rarefactional phase of the ultrasonic wave. SIGNIFICANCE This is a systematic study to investigate the synchronization effect of PUT, which would be significant for further understanding the mechanism and further improving the treatment efficiency of PUT.Interstitial fibrosis is a pathological expansion of the heart's inter-cellular collagen
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