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The Helsinki Declaration's ethical framework guided this cross-sectional study, which investigated seventy women exhibiting amenorrhea. The sample was classified into forty (57.14%) diagnosed with primary amenorrhea (PA), thirty (42.86%) with secondary amenorrhea (SA), and a control group of thirty healthy women with regular menstrual cycles. In performing chromosomal karyotyping, the 2020 ISCN was the reference standard. To determine the characteristics of women with