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HIIT led to an increase in the fluorescence intensity of CD9, CD63, and CD81 positive sEVs across both PPP and PFP groups. Physical activity, according to these data, is correlated with the release of sEVs into the general circulation. Finally, platelet-free plasma is the optimal, representative substance to investigate the behavior of sEVs and their phenotype during exercise. sEVs, the nano-sized components of extracellular vesicles, are composed of proteins, metabolites, lipids, and RNA, and facilitate inter