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We report a simple methodology to prepare nano-porous polysulfone membranes using zwitterionic functionalized silica nanogels with high BSA protein rejection and antifouling properties. The zwitterionic silica precursor was prepared by reacting 1,3-propane sultone with 3-aminopropyl triethoxysilane under an inert atmosphere. The precursor was in situ hydrolyzed and condensed in the polysulfone nanoporous membrane network by one-pot acidic phase inversion. The prepared membranes were characterized to establish their physicochemical natu