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A protocol targeting adherent A549 cells with transiently expressed pathogenic LRRK2 and immortalized murine embryonic fibroblasts with inherent LRRK2 expression, but adjustable for various other cultured cell types is detailed herein. For a complete description of this protocol's operation and practical implementation, please review Fdez et al.1 and Lara Ordonez et al.2. Effective characterization of how novel small molecules function requi