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It is difficult to explain the intricate mechanisms behind syrinx formation and dilation. We studied the roles of ependymal cells lining the central canal to understand how they could prevent syrinx formation and decrease central canal dilation. Using a total of 78 Sprague-Dawley (SD) rats, each afflicted with syringomyelia, we investigated the role of ependymal cells in the dilation of the central canal. Using immunofluorescence, the activation state