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To determine ceftazidime and pyridine simultaneously in human plasma, a liquid chromatography (LC) method with high sensitivity, accuracy, and precision has been developed and validated. Proteins in the plasma samples were precipitated through the application of acetonitrile (ACN). Chromatography, utilizing gradient elution and a Kinetex C18 column (150 mm × 3 mm, 26 m), was used for separation. Mobile phase A comprised a 98