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The RNA-seq and clinical data from elderly GBC patients were examined in this study to determine the genetic makeup and survival-predicting nomograms. From the Gene Expression Omnibus (GEO) database, RNA-seq data from 14 gallbladder cancer cases were collected and categorized by age, initiating gene differential and enrichment analysis. To further clarify the gene expression profile in elderly GBC patients, a Weighted Gene Co-expression Netw